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  • Anti -BrdU Mouse mAb （ABC12069）
  • Anti -BrdU Mouse mAb （ABC12069）
  • Anti -BrdU Mouse mAb （ABC12069）
Anti -BrdU Mouse mAb （ABC12069）
價格：1200.0

產(chǎn)地：ABC

貨號：ABC12069

規(guī)格：100UL

備注：

  • Anti -BrdU Mouse mAb （ABC12069）
  • Anti -BrdU Mouse mAb （ABC12069）
  • Anti -BrdU Mouse mAb （ABC12069）
產(chǎn)品信息
產(chǎn)品名稱 產(chǎn)品編號 規(guī)格
Anti -BrdU Mouse mAb ABC12069 100UL
Product Information
Protein full name Bromodeoxyuridine
Synonyms Bromodeoxyuridine, Bromodeoxyuridine,5-Bromo-2′-deoxyuridine; 5-BrdU, Proliferation Marker; 5-Bromo-2-deoxyUridine
Immunogen KLH conjugated Synthetic peptide corresponding to Mouse BrdU
Isotype IgG1,κ
Purity Affinity purification
Subcellular location Nucleus
Applications
IHC/IF All species 1: 100-1: 500 jejunum, neonatal mouse's brain
Background

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it.

Images
IHC analysis of Brdu (GB12051).
Sample: Mouse jejunum (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: 2M HCL in pure water，37℃, 30minBlocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.
IHC analysis of Brdu (GB12051).
Sample: Mouse jejunum (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: 2M HCL in pure water，37℃, 30minBlocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.
IF analysis of Brdu (GB12051).
Sample: Mouse neonatal mouse's brain (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG (H+L)(GB21301),1: 200 RT 1h.
Storage
Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

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