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  • Anti -Insulin Mouse mAb （ABC12353）
  • Anti -Insulin Mouse mAb （ABC12353）
  • Anti -Insulin Mouse mAb （ABC12353）
  • Anti -Insulin Mouse mAb （ABC12353）
  • Anti -Insulin Mouse mAb （ABC12353）
Anti -Insulin Mouse mAb （ABC12353）
價(jià)格：1200.0

產(chǎn)地：ABC

貨號(hào)：ABC12353

規(guī)格：100UL

備注：

  • Anti -Insulin Mouse mAb （ABC12353）
  • Anti -Insulin Mouse mAb （ABC12353）
  • Anti -Insulin Mouse mAb （ABC12353）
  • Anti -Insulin Mouse mAb （ABC12353）
  • Anti -Insulin Mouse mAb （ABC12353）
產(chǎn)品信息
產(chǎn)品名稱 產(chǎn)品編號(hào) 規(guī)格
Anti -Insulin Mouse mAb ABC12353 100UL
Product Information
Protein full name Insulin-1
Synonyms INS, IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10, insulin
Immunogen Recombinant protein corresponding to Mouse Insulin
Uniprot ID P01325
Isotype IgG2a,κ
Purity Affinity purification
Subcellular location Secreted
Predicted MW. / Observed MW. 12 kDa / 12 kDa
Applications
WB Mouse 1: 1000-1: 2000 pancreas
IHC/IF Human, Mouse, Rat 1: 1000-1: 2000 pancreas
Background

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Images
WB analysis of insulin (GB12335).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 3000, RT, 1h.
IHC analysis of insulin (GB12335).
Sample: Mouse pancreas (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: , 4℃ overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.
IF analysis of insulin (GB12335).
Sample: Human pancreas (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG (H+L)(GB21301),1: 200 RT 1h.
IF analysis of insulin (GB12335).
Sample: Mouse pancreas (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG (H+L)(GB21301),1: 200 RT 1h.
IF analysis of insulin (GB12335).
Sample: Rat pancreas (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG (H+L)(GB21301),1: 200 RT 1h.
Storage
Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

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