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  • Anti -CD8 alpha Mouse mAb （ABC12086）
  • Anti -CD8 alpha Mouse mAb （ABC12086）
  • Anti -CD8 alpha Mouse mAb （ABC12086）
  • Anti -CD8 alpha Mouse mAb （ABC12086）
Anti -CD8 alpha Mouse mAb （ABC12086）
價(jià)格：1200.0

產(chǎn)地：ABC

貨號(hào)：ABC12086

規(guī)格：100UL

備注：

  • Anti -CD8 alpha Mouse mAb （ABC12086）
  • Anti -CD8 alpha Mouse mAb （ABC12086）
  • Anti -CD8 alpha Mouse mAb （ABC12086）
  • Anti -CD8 alpha Mouse mAb （ABC12086）
產(chǎn)品信息
產(chǎn)品名稱 產(chǎn)品編號(hào) 規(guī)格
Anti -CD8 alpha Mouse mAb ABC12086 100UL
Product Information
Protein full name T-cell surface glycoprotein CD8 alpha chain
Synonyms T-lymphocyte differentiation antigen T8/Leu-2, CD8a, MAL, Leu2, Leu2 T lymphocyte antigen, Ly 35, LYT3, p32, T8 T cell antigen
Immunogen KLH conjugated Synthetic peptide corresponding to Human CD8 alpha
Uniprot ID P01732
Isotype IgG1,κ
Purity Affinity purification
Subcellular location Cell membrane, Secreted
Applications
IHC/IF Human 1: 1500-1: 3000 colon cancer, colon, lung cancer
Background

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex.

Images
IHC analysis of CD8A (GB12068).
Sample: Human colon cancer (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 3000, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.
IHC analysis of CD8A (GB12068).
Sample: Human colon (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 3000, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.
IHC analysis of CD8A (GB12068).
Sample: Human lung cancer (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 3000, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.
IHC analysis of CD8A (GB12068).
Sample: Human tonsil (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 3000, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.
Storage
Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

This product is intended for research only.

This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

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