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Anti -beta Catenin Rabbit pAb （ABC11033）

價格：1200.0

產(chǎn)地：ABC

貨號：ABC11033

規(guī)格：100UL

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上一個：Anti -Collagen I Rabbit pAb （ABC11040-3）下一個：暫無

產(chǎn)品信息

產(chǎn)品名稱	產(chǎn)品編號	規(guī)格
Anti -beta Catenin Rabbit pAb	ABC11033	100UL

Product Information

Description	beta Catenin rabbit polyclonal antibody
Protein full name	Catenin beta-1
Synonyms	CTNNB1, CTNNB, MRD19, armadillo, catenin beta 1
Immunogen	KLH conjugated Synthetic peptide corresponding to Mouse β-catenin
Isotype	IgG
Purity	Affinity purification
Subcellular location	Cytoplasm, Nucleus, Cell junction
Predicted MW.	92 kDa
Observed MW.	92 kDa
Uniprot ID	P35222, Q02248, Q9WU82

Applications

WB	Human, Mouse, Rat	1: 500-1: 1000	brain
ICC/IF	Human, Mouse, Rat	1: 500-1: 1000	colon, small intestine, stomach

背景

β-catenin is a dual function protein, involved in regulation and coordination of cell–cell adhesion and gene transcription. In humans, the CTNNB1 protein is encoded by the CTNNB1 gene. In Drosophila, the homologous protein is called armadillo. β-catenin is a subunit of the cadherin protein complex and acts as an intracellular signal transducer in the Wnt signaling pathway. It is a member of the catenin protein family and homologous to γ-catenin, also known as plakoglobin. Beta-catenin is widely expressed in many tissues. In cardiac muscle, beta-catenin localizes to adherens junctions in intercalated disc structures, which are critical for electrical and mechanical coupling between adjacent cardiomyocytes.

Images

	WB analysis of β-catenin (GB11015). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h.
	IHC analysis of β-catenin (GB11015). Sample: Human stomach (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.
	IF analysis of β-catenin (GB11015). Sample: Human colon (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.
	IF analysis of β-catenin (GB11015). Sample: Human stomach (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.
	IF analysis of β-catenin (GB11015). Sample: Mouse small intestine (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 200, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.
	IF analysis of β-catenin (GB11015). Sample: Rat stomach (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 200, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.