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  • Anti -NeuN Rabbit pAb （ABC11156）
  • Anti -NeuN Rabbit pAb （ABC11156）
  • Anti -NeuN Rabbit pAb （ABC11156）
  • Anti -NeuN Rabbit pAb （ABC11156）
Anti -NeuN Rabbit pAb （ABC11156）
價格：1200.0

產(chǎn)地：ABC

貨號：ABC11156

規(guī)格：100UL

備注：

  • Anti -NeuN Rabbit pAb （ABC11156）
  • Anti -NeuN Rabbit pAb （ABC11156）
  • Anti -NeuN Rabbit pAb （ABC11156）
  • Anti -NeuN Rabbit pAb （ABC11156）
產(chǎn)品信息
產(chǎn)品名稱 產(chǎn)品編號 規(guī)格
Anti -NeuN Rabbit pAb ABC11156 100UL
Product Information
Protein full name RNA binding protein fox-1 homolog 3
Synonyms FOX3, HRNBP3, NeuN, RBFOX3
Immunogen KLH conjugated Synthetic peptide corresponding to Mouse NeuN
Uniprot ID Q8BIF2
Isotype IgG
Purity Affinity purification
Subcellular location Nucleus, Cytoplasm
Predicted MW. / Observed MW. 41 kDa / 48 kDa
Applications
Applications Species Dilution Positive Sample
WB Mouse, Rat 1:500-1:1000 brain, cortex, hippocampus
IHC/IF Human, Mouse, Rat 1:500-1:1000 brain, hippocampus
Background

NeuN, encoded by FOX3, is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.

Images
WB analysis of Neun (GB11138).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h.
IHC analysis of neun (GB11138).
Sample: Rat brain (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.
IHC analysis of neun (GB11138).
Sample: Mouse brain (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.
IF analysis of neun (GB11138).
Sample: Rat hippocampus (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.
Storage
Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

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