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Anti -Collagen III Rabbit pAb （ABC111647）

價(jià)格：1200.0

產(chǎn)地：ABC

貨號(hào)：ABC111647

規(guī)格：100UL

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上一個(gè)：Anti -p53 Rabbit pAb （ABC111758）下一個(gè)：Anti -Hsp90 Rabbit pAb （ABC11302-1）

產(chǎn)品信息

產(chǎn)品名稱(chēng)	產(chǎn)品編號(hào)	規(guī)格
Anti -Collagen III Rabbit pAb	ABC111647	100UL

Product Information

Protein full name	Collagen alpha-1(III) chain
Synonyms	COL3A1, EDS4A, collagen type III alpha 1, collagen type III alpha 1 chain
Immunogen	Recombinant protein corresponding to Mouse Collagen III
Uniprot ID	P08121
Isotype	IgG
Purity	Affinity purification
Subcellular location	Secreted
Predicted MW. / Observed MW.	138 kDa / 140-220 kDa

Applications

WB	Mouse, Rat	1: 500-1: 1000	skin
IHC/IF	Human, Mouse, Rat	1: 250-1: 1000	kidney, liver, lung

Background

This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.

Images

	WB analysis of collagen III (GB111629). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h.
	IHC analysis of collagen III (GB111629). Sample: Human kidney (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Pepsin Antigen Repair Solution,37℃,30min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.
	IHC analysis of collagen III (GB111629). Sample: Rat kidney (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Pepsin Antigen Repair Solution,37℃,30min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.
	IHC analysis of collagen III (GB111629). Sample: Rat lung (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Pepsin Antigen Repair Solution,37℃,30min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.
	IF analysis of collagen III (GB111629). Sample: Mouse kidney (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Pepsin Antigen Repair Solution,37℃,30min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.
	IF analysis of collagen III (GB111629). Sample: Mouse lung (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Pepsin Antigen Repair Solution,37℃,30min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.
	IF analysis of collagen III (GB111629). Sample: Rat liver (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Pepsin Antigen Repair Solution,37℃,30min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.
	IF analysis of collagen III (GB111629). Sample: Rat lung (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Pepsin Antigen Repair Solution,37℃,30min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.