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  • Anti -p53 Rabbit pAb (ABC111758)
  • Anti -p53 Rabbit pAb (ABC111758)
  • Anti -p53 Rabbit pAb (ABC111758)
  • Anti -p53 Rabbit pAb (ABC111758)
  • Anti -p53 Rabbit pAb (ABC111758)
Anti -p53 Rabbit pAb (ABC111758)
價格:1200.0

產(chǎn)地:ABC

貨號:ABC111758

規(guī)格:100UL

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  • Anti -p53 Rabbit pAb (ABC111758)
  • Anti -p53 Rabbit pAb (ABC111758)
  • Anti -p53 Rabbit pAb (ABC111758)
  • Anti -p53 Rabbit pAb (ABC111758)
  • Anti -p53 Rabbit pAb (ABC111758)
產(chǎn)品信息
產(chǎn)品名稱 產(chǎn)品編號 規(guī)格
Anti -p53 Rabbit pAb ABC111758 100UL
Product Information
Description p53 rabbit polyclonal antibody
Protein full name Cellular tumor antigen p53
Synonyms TP53, BCC7, LFS1, P53, TRP53, tumor protein p53
Immunogen Recombinant protein corresponding to Human p53
Isotype IgG
Purity Affinity purification
Subcellular location Nucleus, Endoplasmic reticulum, Mitochondrion
Predicted MW. 44 kDa
Observed MW. 55 kDa
Uniprot ID P04637
Applications
WB Human 1: 1000-1: 2000 HT-29, 293T, HEK293, SW480, U-251MG
IHC/IF Human 1: 200-1: 1000 cervical cancer, colon cancer
Background

Tumor protein p53, also known as p53, cellular tumor antigen p53 , phosphoprotein p53, tumor suppressor p53, antigen NY-CO-13, or transformation-related protein 53 (TRP53), which encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, sense cence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome.

Images
WB analysis of P53 (GB111740).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h.
IHC analysis of P53 (GB111740).
Sample: Human cervical cancer (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.
IHC analysis of P53 (GB111740).
Sample: Human colon cancer (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.
IHC analysis of P53 (GB111740).
Sample: Human colon cancer (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.
IHC analysis of P53 (GB111740).
Sample: Human esophageal cancer (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.
Storage
Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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