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Anti -GAPDH Rabbit pAb （ABC11020）

價(jià)格：200.0

產(chǎn)地：ABC

貨號(hào)：ABC11020

規(guī)格：100UL

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產(chǎn)品信息

產(chǎn)品名稱	產(chǎn)品編號(hào)	規(guī)格
Anti -GAPDH Rabbit pAb	ABC11020	100UL

Product Information

Description	GAPDH rabbit polyclonal antibody
Protein full name	Glyceraldehyde-3-phosphate dehydrogenase
Synonyms	GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase
Immunogen	KLH conjugated Synthetic peptide corresponding to Mouse GAPDH
Isotype	IgG
Purity	Affinity purification
Subcellular location	Cytoplasm, Nucleus, Membrane
Predicted MW.	36 kDa
Observed MW.	36 kDa
Uniprot ID	P16858

Applications

WB	Human, Mouse, Rat	1: 1000-1: 2000	brain, spleen, kidney
IHC/IF	Human, Mouse, Rat	1: 500-1: 1000	kidney, testis

Background

Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation ofapoptosis,ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.In sperm, a testis-specific isoenzyme GAPDHS is expressed.

Images

	WB analysis of GAPDH (GB11002). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h.
	WB analysis of GAPDH (GB11002). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h.
	IHC analysis of GAPDH (GB11002). Sample: Mouse kindey (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.
	IHC analysis of GAPDH (GB11002). Sample: Rat testis (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.