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  • Anti -Iba1 Rabbit pAb (ABC113520)
  • Anti -Iba1 Rabbit pAb (ABC113520)
  • Anti -Iba1 Rabbit pAb (ABC113520)
  • Anti -Iba1 Rabbit pAb (ABC113520)
  • Anti -Iba1 Rabbit pAb (ABC113520)
  • Anti -Iba1 Rabbit pAb (ABC113520)
  • Anti -Iba1 Rabbit pAb (ABC113520)
  • Anti -Iba1 Rabbit pAb (ABC113520)
  • Anti -Iba1 Rabbit pAb (ABC113520)
Anti -Iba1 Rabbit pAb (ABC113520)
價(jià)格:1200.0

產(chǎn)地:ABC

貨號(hào):ABC113520

規(guī)格:100UL

備注:

  • Anti -Iba1 Rabbit pAb (ABC113520)
  • Anti -Iba1 Rabbit pAb (ABC113520)
  • Anti -Iba1 Rabbit pAb (ABC113520)
  • Anti -Iba1 Rabbit pAb (ABC113520)
  • Anti -Iba1 Rabbit pAb (ABC113520)
產(chǎn)品信息
產(chǎn)品名稱 產(chǎn)品編號(hào) 規(guī)格
Anti -Iba1 Rabbit pAb ABC113520 100UL
Product Information
Protein full name Allograft inflammatory factor 1
Synonyms AIF-1, AIF1, G1, IBA1, IRT 1, Protein G1, Ionized calcium-binding adapter molecule 1, MRF1, Microglia response factor, BART 1, AIF1 protein
Immunogen Recombinant protein corresponding to Mouse Iba1
Uniprot ID O70200
Isotype IgG
Purity Affinity purification
Subcellular location Cell membrane, Cytoplasm, Cytoskeleton, Cell projection
Applications
IHC/IF Human, Mouse, Rat 1: 500-1: 1000 oligodendroglioma, brain, cerebellum
Background

AIF1, also named as G1, IBA1 or daintain/AIF-1, is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Despite a lack of detailed knowledge on the in vivo physiological functions of AIF-1, there is growing evidence that shows its aberrant expression contributes to the pathogenesis of many autoimmune diseases, including rheumatoid arthritis. AIF1 is an actin-binding protein that enhances membrane ruffling and RAC activation. It enhances lymphocyte migration and the actin-bundling activity of LCP1.

Images
IHC analysis of IBA-1 (GB113502).
Sample: Human oligodendroglioma (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.
IHC analysis of IBA-1 (GB113502).
Sample: Mouse brain (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.
IHC analysis of IBA-1 (GB113502).
Sample: Rat brain (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.
IHC analysis of IBA-1 (GB113502).
Sample: Rat cerebellum (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.
IF analysis of IBA-1 (GB113502).
Sample: Human oligodendroglioma (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.
IF analysis of IBA-1 (GB113502).
Sample: Mouse brain (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.
IF analysis of IBA-1 (GB113502).
Sample: Rat brain (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.
IF analysis of IBA-1 (GB113502).
Sample: Mouse cerebellum (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.
IF analysis of IBA-1 (GB113502).
Sample: Rat cerebellum (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.
Storage
Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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