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  • Anti -GFAP Rabbit pAb （ABC11114）
  • Anti -GFAP Rabbit pAb （ABC11114）
  • Anti -GFAP Rabbit pAb （ABC11114）
  • Anti -GFAP Rabbit pAb （ABC11114）
  • Anti -GFAP Rabbit pAb （ABC11114）
  • Anti -GFAP Rabbit pAb （ABC11114）
Anti -GFAP Rabbit pAb （ABC11114）
價(jià)格：1200.0

產(chǎn)地：ABC

貨號(hào)：ABC11114

規(guī)格：100UL

備注：

  • Anti -GFAP Rabbit pAb （ABC11114）
  • Anti -GFAP Rabbit pAb （ABC11114）
  • Anti -GFAP Rabbit pAb （ABC11114）
  • Anti -GFAP Rabbit pAb （ABC11114）
  • Anti -GFAP Rabbit pAb （ABC11114）
產(chǎn)品信息
產(chǎn)品名稱 產(chǎn)品編號(hào) 規(guī)格
Anti -Iba1 Rabbit pAb ABC11114 100UL
Product Information
Description GFAP rabbit polyclonal antibody
Protein full name Glial fibrillary acidic protein
Synonyms GFAP, ALXDRD, glial fibrillary acidic protein, Astrocyte, Intermediate filament protein
Immunogen KLH conjugated Synthetic peptide corresponding to Mouse GFAP
Isotype IgG
Purity Affinity purification
Subcellular location Cytoplasm
Predicted MW. 49 kDa
Observed MW. 49 kDa
Uniprot ID P14136, P03995, P47819
Applications
WB Human, Mouse, Rat 1: 1000-1: 2000 brain, cerebellum, cortex
IHC/IF Human, Mouse, Rat 1: 500-1: 3000 brain, cerebellum
Background

Glial fibrillary acidic protein is an intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS) including astrocytes and ependymal cells.GFAP has also been found to be expressed in glomeruli and peritubular fibroblasts taken from rat kidneys Leydig cells of the testis in both hamsters and humans, human keratinocytes, human osteocytes and chondrocytes and stellate cells of the pancreas and liver in rats. It is closely related to its non-epithelial family members, vimentin,desmin, and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton.

Images
WB analysis of GFAP (GB11096).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h.
IHC analysis of GFAP (GB11096).
Sample: Rat brain (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 3000, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.
IHC analysis of GFAP (GB11096).
Sample: Mouse brain (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 3000, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.
IHC analysis of GFAP (GB11096).
Sample: Rat cerebellum (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 2800, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.
IF analysis of GFAP (GB11096).
Sample: Mouse brain (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.
IF analysis of GFAP (GB11096).
Sample: Rat brain (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.
Storage
Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

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